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KMID : 0620920230550050974
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Experimental & Molecular Medicine
2023 Volume.55 No. 5 p.974 ~ p.986
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Clinical molecular subtyping reveals intrinsic mesenchymal reprogramming in gastric cancer cells
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Jang Eun-Ji
Shin Min-Kyue
Kim Hyun-Ki
Lim Joo-Yeon
Christopher Chee Kong Ho
Park Jung-Min
Kim Jung-Eun
Kim Hye-Seon
Shin Young-Min
Son Hye-Young
Choi Yoon-Young
Hyung Woo-Jin
Luca Boeri
Suh Jin-Suck
Sung Ji-Yong
Huh Yong-Min
Cheong Jae-Ho
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Abstract
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The mesenchymal cancer phenotype is known to be clinically related to treatment resistance and a poor prognosis. We identified gene signature-based molecular subtypes of gastric cancer (GC, n?=?547) based on transcriptome data and validated their prognostic and predictive utility in multiple external cohorts. We subsequently examined their associations with tumor microenvironment (TME) features by employing cellular deconvolution methods and sequencing isolated GC populations. We further performed spatial transcriptomics analysis and immunohistochemistry, demonstrating the presence of GC cells in a partial epithelial-mesenchymal transition state. We performed network and pharmacogenomic database analyses to identify TGF-β signaling as a driver pathway and, thus, a therapeutic target. We further validated its expression in tumor cells in preclinical models and a single-cell dataset. Finally, we demonstrated that inhibition of TGF-β signaling negated mesenchymal/stem-like behavior and therapy resistance in GC cell lines and mouse xenograft models. In summary, we show that the mesenchymal GC phenotype could be driven by epithelial cancer cell-intrinsic TGF-β signaling and propose therapeutic strategies based on targeting the tumor-intrinsic mesenchymal reprogramming of medically intractable GC.
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KEYWORD
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Cancer genomics, Cancer microenvironment, Classification and taxonomy, Gastric cancer, Tumour heterogeneity
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